biotinylate
biotinylate.01 - attach a biotin residue to a protein or other molecule
Aliases:
biotinylate (v.)
biotinylation (n.)
biotinylation (n.)
Roles:
ARG0-PAG: intentional agent of biotinylation (e.g. scientist)
ARG1-PPT: thing biotinylated (e.g. protein)
ARG2-MNR: instrument
ARG3-PRD: result of biotinylation
ARG1-PPT: thing biotinylated (e.g. protein)
ARG2-MNR: instrument
ARG3-PRD: result of biotinylation
intentional agent
To facilitate the discrimination between human serum proteins and immunoprecipitated antigens
weARG0
biotinylatedrel
proteins of liver cytosolARG1
.example with instrument
The purified oligonucleosomesARG1
arerel
then biotinylatedrel
with an amine - reactive reagentARG2
, before dialysis into the final storage buffer .product of reaction
Thus ,
the 264scTCR / birA fusion proteinARG1
can berel
biotinylatedrel
to create 264scTCR / birA monomersARG3
, which can subsequently be used to form 264scTCR / multimers in the presence of streptavidin .