Rolesets - biotinylate

biotinylate

biotinylate.01 - attach a biotin residue to a protein or other molecule

Aliases:

biotinylate (v.)
biotinylation (n.)

Roles:

ARG0-PAG: intentional agent of biotinylation (e.g. scientist)
ARG1-PPT: thing biotinylated (e.g. protein)
ARG2-MNR: instrument
ARG3-PRD: result of biotinylation

intentional agent

To facilitate the discrimination between human serum proteins and immunoprecipitated antigens
weARG0
biotinylatedrel
proteins of liver cytosolARG1
.

example with instrument

The purified oligonucleosomesARG1
arerel
then
biotinylatedrel
with an amine - reactive reagentARG2
, before dialysis into the final storage buffer .

product of reaction

Thus ,
the 264scTCR / birA fusion proteinARG1
can
berel
biotinylatedrel
to create
264scTCR / birA monomersARG3
, which can subsequently be used to form 264scTCR / multimers in the presence of streptavidin .