Rolesets - dephosphorylate

dephosphorylate

dephosphorylate.01 - remove a phosphate group from an organic compound by hydrolysis

DEPHOSPHORYLATE-V NOTES: Created for Bio-AMR annotations.

Aliases:

dephosphorylate (v.)
dephosphorylation (n.)

Roles:

ARG0-PAG: intentional agent of dephosphorylation (e.g. scientist)
ARG1-PPT: thing dephosphorylated
ARG2-MNR: instrument
ARG3-PRD: product of dephosphorylation

dephosphorylate-v: passivized, with product of the reaction

Regarding the relationship between adenosine and ATPARGM-ADV
,
intracellular adenosineARG3
is
dephosphorylatedrel
from AMPARG1
by cytosolic 5'-nucleotidaseARG2
and is converted back to AMP via adenosine kinase ( ADK ) .

dephosphorylate-v: agent and instrument

For cloningARGM-PRP
,
weARG0
dephosphorylaterel
the RNAsARG1
with shrimp nucleaseARG2
and deproteinate the reaction with proteinase K , followed by phenol / chloroform extraction .

dephosphorylation-n

Dephosphorylationrel
of the 5 ' endARG1
prohibits self - ligation , enabling the researcher to manipulate the DNA as desired before re - ligating .