dephosphorylate
dephosphorylate.01 - remove a phosphate group from an organic compound by hydrolysis
DEPHOSPHORYLATE-V NOTES: Created for Bio-AMR annotations.Aliases:
dephosphorylate (v.)
dephosphorylation (n.)
dephosphorylation (n.)
Roles:
ARG0-PAG: intentional agent of dephosphorylation (e.g. scientist)
ARG1-PPT: thing dephosphorylated
ARG2-MNR: instrument
ARG3-PRD: product of dephosphorylation
ARG1-PPT: thing dephosphorylated
ARG2-MNR: instrument
ARG3-PRD: product of dephosphorylation
dephosphorylate-v: passivized, with product of the reaction
Regarding the relationship between adenosine and ATPARGM-ADV
, intracellular adenosineARG3
is dephosphorylatedrel
from AMPARG1
by cytosolic 5'-nucleotidaseARG2
and is converted back to AMP via adenosine kinase ( ADK ) .dephosphorylate-v: agent and instrument
For cloningARGM-PRP
, weARG0
dephosphorylaterel
the RNAsARG1
with shrimp nucleaseARG2
and deproteinate the reaction with proteinase K , followed by phenol / chloroform extraction .dephosphorylation-n
Dephosphorylationrel
of the 5 ' endARG1
prohibits self - ligation , enabling the researcher to manipulate the DNA as desired before re - ligating .