Rolesets - subclone

subclone

subclone.01 - reclone part of a previously-cloned DNA segment into a new vector

Aliases:

subclone (v.)
subclone (n.)

Roles:

ARG0-PAG: intentional agent of subcloning (e.g. scientist)
ARG1-PPT: cloned entity
ARG2-DIR: source, parent vector
ARG3-GOL: destination vector

intentional agent

WeARG0
subclonedrel
two chicken H3 histone genesARG1
and transfected them into Rat 3 cell line .

parent and destination vectors

The gene encoding ɸ8 P4 helicaseARG1
wasrel
subclonedrel
from pET32ARG2
into pET28bARG3
for incorporation of an N - terminal His tag ( pET28b - P4 - His ) .